Protein Modifiers

SATA
(NSuccinimidylSacetylthioacetate)
Adds protected sulfhydryls for better control when a sulfhydryl group is needed in conjugate formation.

M.W. 231.23
Spacer Arm 2.8 Â

Features/Benefits:
- Reacts with amines to add protected sulfhydryl groups
- NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
- Converts amino groups to sulfhydryl groups
- Latent -SH group is released by hydroxylamine and is available for reaction with maleimide- activated biomolecules or other -SH group-containing compounds
- Cross-links formed with other -SH group-containing molecules are reversible by reducing agents
- Reactive groups: NHS ester and thioacetylprotected sulfhydryl
- Reactive toward: amino and maleimide/iodoacetyl or vinyl sulfone

Reference: Duncan, R.J.S., et al. (1983) Anal. Biochem. 132, 66-73