Campbell Science synthesizes the most colorless and pure reagents currently available. Materials produced in our laboratory are shipped worldwide.
Campbell Science was founded in 1996. Our focus is on providing high purity reagents at a reasonable cost and minimizing back orders through coordinated production management. We also have dedicated ourselves tointroducing new and innovative products to the market place.
We have added several new product lines including the Enzyme Beta-Glucuronidase, Cross-Linking Reagents, Protein Modifiers, and High Purity Detergents. We are working on several new product lines for the future.
Sulfo-NHS-LC-Biotin (Sulfosuccinimidyl-6-(biotinamido)hexanoate)
Features/Benefits
- Water-soluble analog of NHS-LC-Biotin
- Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phoshate buffers
- Negative charge of -SO 3 group keeps biotinylation localized at the cell surfaces so reagent does not pass through cell membrane
- Sulfo-NHS ester group will start to hydolyze once it is dissolved in an aqueous buffer
Sulfo-NHS-SS-Biotin (Sulfosuccinimidyl-2-(biotinamido)ethyl-1,3-dithiopropionate)
Features/Benefits
- Water-soluble and cleavable analog of NHS-LC-Biotin
- Ideal for reversible biotinylation, biotin separated from molecule when spacer arm is cleaved
- Extended spacer arm prevents steric hindrance
NHS-Biotin (N-Hydroxysuccinimidobiotin)
Features/Benefits
- Shorter spacer arm than Sulfo-NHS-LC-Biotin
- Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
- Negative charge of -SO 3 keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
- Eliminates the need for harmful organic solvents
Sulfo-NHS-Biotin (N-Hydroxysulfosuccinimidobiotin)
Features/Benefits
- Forms stable amide bonds
- Reacts with primary amines at pH 7-9
- Must be dissolved in DMSO or DMF and can then be transferred to an aqueous reaction solution.
- Penetrates cell membranes
Biotin Hydrazide
Features/Benefits
- Carbohydrate-reactive
- Must be dissolved in DMSO (50 mM) before adding to aqueous buffer
- Use at pH 4-6 to complete coupling reaction
NHS-Bromoacetate (N-Hydroxysuccinimidyl Bromoacetate)
NHS-lodoacetate (N-Hydroxysuccinimidyl lodoacetate)
Sulfo-LC-SPDP (Sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido] hexanoate)
Features/Benefits
- Sulfo-LC-SPDP releases a detectable byproduct after reacting with free sulfhydryl groups, by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Reactive groups pyridyidithio and Sulfo-NHS ester
- Reactive toward sulfhydryl and amino groups
LC-SPDP (Succinimidyl 6-[2-pyridyldithio)propionamido]hexanoate)
Features/Benefits
- LC-SPDP releases a detectable byproduct after reacting with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Reactive groups: pyridyldithio and NHS ester
- Reactive toward: sulfhydryl and amino groups
SPDP (N-Succinimidyl-3-(2-pyridyldithio)propionate)
Features/Benefits
- Widely used immunochemistry; conjugates used in drug carrier systems, antibody productin and enzyme immunoasays have been successfully prepared with SPDP
- SPDP can be used a s a protein thiolatin reagent, resulting in available -SH groups
- Reactive groups: NHS ester and pyridyldithio
- Reactive toward: amino and sulfhydryl groups
BSSS (Bis(sulfosuccinimidyl)suberate)
Sulfo-EGS (Ethylene Glycol bis(Sulfosuccinimidylsuccinate))
Features/Benefits
- Cross-links formed are cleavable at pH 8.5 using hydroxylamine for three to six hours at 37 degree C
- Lactose dhydrogenase retained 60% of its activity after reversible cross-linking with EGS
- Reactive groups: Sulfo-NHS esters (homobifunctional)
- Reactive toward: amino groups
EGS (Ethylene Glycol bis(Succinimidylsuccinate))
Features/Benefits
- Cross-links formed are cleavable at pH 8.5 using hydroxylamine for three to six hours at 37 degrees C
- Lactos dehydrogenase retained 60% of its activity after reversible cross-linking with EGS
- Reactive groups: NHS esters (homobifunctinal)
- Reactive toward: amino groups
DTSSP (3,3'Dithiobis(sulfosuccinimidylpropionate))
Features/Benefits
- Conditions for reducing the disulfide bond include: 50mM DTT, 100mM B-mercaptoethanol or 1% sodium borohydride
- Reactive groups: Sulfo-NHS esters (homobifunctional)
- Reactive toward: amino groups
DSS (Dissuccinimidyl Suberate)
Features/Benefits
- Used for conjugating a radiolabeled ligand to a cell surface receptor
- Water-insoluble; non-cleavable
- Reactive groups: NHS ester (homobifunctional)
- Reactive toward: amino groups
BSSS (Bis(sulfosuccinimidyl)suberate)
Features/Benefits
- Water-soluble DSS analog
- Non-cleavable
- Membrane-impermeable
- Reactive groups: Sulfo-NHS ester (homobifunctional)
- Reactive toward: amino groups
CHAPS (3-[(3-Cholamidopropyl)Dimethylammonio]-2-Hydroxy-1-propanesulfonate)
Features/Benefits
- Combine all the advantages of polar, sulfobetaine-containing detergents and hydrophobic, bile salt, anionic detergents into a single molecule with superior membrane protein solubilization properties
- Non-denaturing
- Able to disrupt nonspecific protein interactions
- Less protein aggregation than nonionic detergents
- Electrically neutral
- Easily removed by dialysis
CHAPSO (3-[(3-Cholamidopropyl)Dimethylammonio]-2-Hydroxy-1-propanesulfonate)
- Zwitterionic detergent Non-denaturing
TMAH - 0.2 M (Trimethylanilinium Hydroxide Methanol Solution)
Flash methylation reagent
4-CB (4-Carbethoxyhexafluorobutyryl chloride)
99% purity
Molecular Weight - 271.
Boiling point - 159C.
CAS# [18381-53-8]
Density - 1.596.
Derivatizes amphetamine metabolites
HFIP (1,1,1,3,3,3-Hexafluoro-2-propanol)
99.5% purity
Molecular Weight - 168.
Boiling Point -59C.
CAS# [920-66-1]
Density - 1.596.
Esterification reagent
PFPOH (2,2,3,3,3-Pentafluoro-1-propanol)
99% purity
Molecular Weight - 150.
Boiling Point -80C.
CAS# [422-05-09]
Density - 1.505.
Esterification reagent
TFAA (Trifluroacetic Anhydride)
99% purity
Molecular weight - 210.
Boiling Point 40C.
CAS# [407-25-0]
Density - 1.487
Acylates alcohols, phenols and amines.
MBTFA (N-Methyl-bis(trifluoroacetamide))
99% purity
Molecular Weight - 223.
Boiling Point -122C.
CAS# [685-27-8]
Density - 1.55.
Acylates hydroxyls, thiols, primary and secondary amines.
HFBA (Heptafluorobutyric Anhydride)
99% purity
Molecular Weight -410.
Boiling Point -108C.
CAS# [336-59-4]
Density - 1.665.
Acylates alcohols, phenols and amines.
PFPA (Pentafluoropropionic Anhydride)
99% purity
Molecular Weight - 310.
Boiling Point -74C.
CAS# [356-42-3]
Density - 1.571
Acylates alcohols, phenols and amines.
BSTFA + 1% TMCS (N,O-bis(rimethylsilyl) trifluoroacetamide)
Molecular Weight - 257.
Boiling Point -145C.
[25561-30-2]
Density - 0.974.
Silylates hydroxyls, carboxyls, amines, amides, and steroids.
BSTFA (N,O-bis(rimethylsilyl) trifluoroacetamide)
98% purity
Molecular Weight - 257.
Boiling Point -145C.
[25561-30-2]
Density - 0.974.
Silylates hydroxyls, carboxyls, amines, amides, and steroids.
MTBSTFA + 1% TBDMCS (N-(tert-butyldimethylsilyl)-N-methltrifluoroacetamide)
Molecular Weight - 241.
Boiling Point -174C.
[77377-52-7]
Density - 1.036.
Silylates hydroxyls, carboxyls, thiols, primary and secondary amines.
MTBSTFA (N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide)
Molecular Weight - 241.
Boiling Point -174C.
[77377-52-7]
Density - 1.036.
Silylates hydroxyls, carboxyls, thiols, primary and secondary amines.
MSTFA (N-Methyl-N-(trimethylsilyl) trifluoroacetamide)
98% purity
Molecular Weight - 199.
Boiling Point -132C.
[24589-78-4]
Density - 1.08.
MSTFA +1% TMCS (N-Methyl-N-(trimethylsilyl) trifluoroacetamide)
Molecular Weight - 199.
Boiling Point -132C.
[24589-78-4]
Density - 1.08.
Silylates hydroxyls, carboxyls, amines, amides, and steroids.
B-glucuronidase Solution
100,000 Units Betagluccoronidase per ml in deionized water - min 7000 units per ml Sulfatase
B-glucuronidase (freeze dried from abalone entrails)
B-D-Glucuronide glucuronosohydrolase
One unity will liberate 1.0 phenolphthalein from phenolphthalein glucuronide per hour at 37C.
SATA (N-Succinimidyl-S-acetylthioacetate)
Features/Benefits:
- Reacts with amines to add protected sulfhydryl groups
- NHS ester and couples with primary amines at pH 7-9 to form stable amide bonds
- Converts amino groups to sulfhydryl groups
- Latent -SH group is released by hydroxylamine and is available for reaction with maleimide- activated biomolecules or other -SH group-containing compounds
- Cross-links formeed with other -SH group-containing molecules are reversible by reducing agents
- Reactive groups: NHS ester and thioacetylprotected sulfhydryl
- Reactive toward: amino and maleimide/iodoacetyl or vinyl sulfone
TCEP (Tris(2-carboxyethyl)phosphine Hydrochloride)
Potent, water-soluble, odorless reducing agent in a conventional solid format.
Features/Benefits:
- Selective and complete reduction of even the most stable water-soluble alkyl disulfides
- Effective reduction at room temperature at pH 5 in less than five minutes
- Water solubility of 310 gm/L
- Resistant to air oxidation; nonvolatile and nonreactive toward other functional groups found in proteins