Cross-linking Reagents

LC-SPDP
(Succinimidyl 6-[3-2-pyridyldithio)propionamido]hexanoate)
Classic heterobifunctional, cleavable cross-linker, with an extended arm.

M.W. 425.52
Spacer Arm 15.7 Â

Features/Benefits:
- LC-SPDP releases a detectable byproduct after reacting with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Reactive groups: pyridyldithio and NHS ester
- Reactive toward: sulfhydryl and amino groups

Reference: Carlsson, J., et al. (1978) Biochem. J. 173, 723-737