Biotinylation Reagents

Sulfo-NHS-Biotin
(NHydroxysulfosuccinimidobiotin)
Water soluble analog of NHS-Biotin

M.W. 443.43
Spacer Arm 13Â

Features/Benefits:
- Shorter spacer arm than Sulfo-NHS-LC-Biotin
- Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
- Negative charge of -SO 3 keeps Biotinylation localized at the cell surface so reagent does not pass through cell membrane
- Eliminates the need for harmful organic solvents

REFERENCE: LEE, W.T. and CONRAD, D.H. (1984) J.Exp. Med. 159, 1790-1795